Barca had sought after a moment incredible escape after they toppled a 4-0 first-leg deficiency against Paris Saint-Germain in the Last 16, yet the Italians demonstrated an alternate class by totally fixing the fearsome assault of Lionel Messi, Luis Suarez and Neymar in a protective masterclass.
Juventus, who picked up reprisal for their annihilation to Barca in the 2015 Champions League last, join holders Real Madrid, Atletico Madrid and Monaco in Friday's elimination round draw.
Juve demonstrated awesome strategic sagacious by squeezing high and anticipating Barca commanding the ball as they did against PSG, in spite of the fact that Messi ought to have improved his shot from the edge of the territory following 19 minutes.
The Argentine maestro then stung Gianluigi Buffon's palms interestingly on the half-hour check before hitting the side-netting on the development.
At the flip side, Higuain and Juan Cuadrado verged on breaking Barca hearts with a pivotal away objective however did not have the obliged completing to pulsate Marc-Andre ter Stegen.
Neymar, who started the rebound against PSG cut a disappointed figure as official Bjorn Kjuipers permitted various powerful difficulties go unpunished while Messi needed to go further looking for the slippery opener.
Barca mentor Luis Enrique tossed on Paco Alcacer as the hosts played with four advances for the last half hour, their most obvious opportunity tumbling to Messi after an uncommon Buffon mistake from a corner, however sadly he skied the exertion with his correct boot.
Juventus expertly observed out the last stages to enroll their eighth clean sheet in 10 Champions League amusements this season as Barca bow out of the race.
Somewhere else, Monaco beat Borussia Dortmund 3-1 in the second-leg of their last eight conflict Wednesday to advance 6-3 on total.
Dortmund captain Marco Reus split his side's shortage right on time in the second half after Kylian Mbappe and Radamel Falcao had put the hosts ahead, yet substitute Valere Germain put the seal on their triumph as Monaco experience to the semis surprisingly since 2004.